Layouts in OBO-Edit are referred to as Perspectives. OBO-Edit offers several built-in perspectives and also allows users to customize their own perspectives (which can then be saved and shared with other users). The menu options for perspectives are in the Layouts menu.
There are two built-in perspectives called Edit and Verify. The Edit perspective is optimized for ontology editing:

The Verify perspective allows you to easily check various features of the ontology such as comment syntax:
In addition to these built-in perspectives you can create your own favorite perspective, save it, and (if desired) share the resulting configuration file with other people. You can set up your desired perspective by using the menus to add components and/or the "X" icons on currently displayed components to remove them from the display.
Components can be moved around by grabbing their title bars and dragging them around. As you drag a panel, you will see a black outline that shows where it will land if you drop it.
You can turn a panel into an independent window (i.e., undock it) by clicking the icon of an arrow that points upwards and to the right:

To return a panel to the main OBO-Edit window (i.e., dock it), click its icon that shows an arrow pointing downwards and to the left.
First set up your perspective by adding or removing panels and dragging them to your preferred locations. Then save the perspective (using [Layouts]->[Save current perspective as..]). (Your perspective will now show up in the list of perspectives when you access the Layouts menu.)
To find your perspectives file to share with other users, go to your configuration directory and look inside the perspectives subdirectory for the appropriate .idw file. If you don't know where your configuration directory is, you can find out by opening the Configuration Manager. It is not possible to edit the perspectives file by hand, but you can mail the file around to let others use your favorite perspective.To import a perspective, use [Layout] -> [Import perspective...]. The perspective name will be derived from the filename (e.g., if the file is called "new-perspective.idw", the perspective will be called "new-perspective").